Tests and Studies: Chromatography

Chromatography is used to identify the range of colour in the leaf and takes about 27 hours.

What do we need?

  • Healthy, green leaves
  • Jam jar
  • Pestle and mortar
  • Nail varnish remover
  • Kitchen paper
  • Pen

How do we do it?

  1. Take a few single leaves and place them in the mortar.
  2. Using a twisting action, pound down on the leaves with the pestle to remove some of the leaf’s moisture and to break down the leaf’s walls.
  3. Places the crushed leaves in a jam jar.
  4. Pour the nail varnish remover (which contains acetone) on top.
  5. Place the lid on top of the jar and shake.
  6. After 24 hours wrap a piece of kitchen paper around a pen so that some kitchen paper reaches the liquid in the jar when the pen is placed horizontally over the top of the jar.
  7. Leave for 2 hours.
  8. Monitor the colour on the kitchen paper.

My results?

This leaf just contained a few levels of the colour green.

Categories: Tests and Studies

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